A CAPE approach to gamma-Linolenic acid production via lipase-catalyzed enzymatic hydrolysis

In this work, a lipase-catalyzed selective enzymatic hydrolysis of the borage oil was carried out using commercial enzymes and native crude enzymatic extract from Brazil as biocatalysts for the reaction. Two crude enzymatic extracts home-produced and three commercial lipases were screened for concentrating γlinolenic acid of the triacylglycerols in unhydrolyzed acylglycerols. The kinetics of enzymatic hydrolysis of borage oil using Geotrichum candidum lipase in a batch reactor has been investigated. This work also presents a systematic procedure for parameter estimation through of dynamic optimization implemented as a nonlinear programming problem. With the reaction rate, a simulator was developed to explore the possible operational strategies for a large-scale reactor. The proposed computer-aided tool was applied to optimize the parameter of Michaelis-Menten kinetic model. 2 P. B. L. Fregolente et al.